Characterization of murine A-raf, a new oncogene related to the v-raf oncogene.
Open Access
- 1 July 1986
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 6 (7) , 2655-2662
- https://doi.org/10.1128/mcb.6.7.2655
Abstract
A 1.6-kilobase cDNA (A-raf) has been isolated from a murine spleen cDNA library which encodes part of a protein related to the raf oncogene. Its amino acid sequence has 85% homology to raf in a central portion of 100 amino acids. In contrast to raf, A-raf shows a highly restricted tissue distribution of expression, with highest levels observed in epididymis, followed by intestine. When incorporated into a retrovirus, the resulting gag-A-raf fusion gene causes transformation in vitro and induces tumors in newborn mice. Thus, A-raf represents a new proto-oncogene. Transformation by A-raf is independent of ras gene function, as is the case for raf and mos but not other oncogenes.This publication has 46 references indexed in Scilit:
- The neu Gene: an erb B-Homologous Gene Distinct from and Unlinked to the Gene Encoding the EGF ReceptorScience, 1985
- Amplification of a Novel v- erb B-Related Gene in a Human Mammary CarcinomaScience, 1985
- Activation of the transformation potential of the cellular fps geneCell, 1985
- Induction of proliferation or transformation of neuroretina cells by the mil and myc viral oncogenesNature, 1985
- An N-terminal peptide from p60src can direct myristylation and plasma membrane localization when fused to heterologous proteinsNature, 1985
- Serine- and threonine-specific protein kinase activities of purified gag–mil and gag–raf proteinsNature, 1984
- Nucleotide sequence of avian retroviral oncogene v-mil: homologue of murine retroviral oncogene v-rafNature, 1984
- The Human Homologs of the raf ( mil ) Oncogene Are Located on Human Chromosomes 3 and 4Science, 1984
- Homologous cell-derived oncogenes in avian carcinoma virus MH2 and murine sarcoma virus 3611Nature, 1984
- Transformation of MMC-E epithelial cells by acute 3611-MSV: inhibition of collagen synthesis and induction of novel polypeptidesJournal of Cellular Biochemistry, 1982