Pyridine Derivatives Stimulate Phosphatidylcholine Secretion in Primary Cultures of Rat Type II Pneumocytes

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Abstract
We have examined the effects of pyridine derivatives on phosphatidylcholine secretion in primary cultures of rat type II pneumocytes. Of 12 pyridine derivatives, 4−aminopyridine, 4−dimethylaminopyridine and 4−pyrolidinopyridine had a stimulatory effect on phosphatidylcholine secretion, whereas other derivatives had little effect. The stimulatory effect of 4−aminopyridine was concentration- and time-dependent, and was inhibited by the acetoxymethyl ester of 1,2−bis(2−aminophenoxy)ethane-N, N, N', N'-tetraacetic acid (3 μM), an intracellular Ca2+ chelator. In addition, the stimulatory effect of 4−aminopyridine was suppressed by W−7 (N-(6−aminohexyl)−5−chloro−1−napthalene-sulphonamide) (10 μM), a calmodulin inhibitor, and sphingosine (10 μM) and staurosporine (01μM), protein kinase C inhibitors. These results indicate that several pyridine derivatives stimulate phosphatidylcholine secretion in type II pneumocytes.

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