SECRETION OF PROTEINS, ANDROGEN BINDING PROTEIN AND OESTRADIOL BY SERTOLI CELLS IN CULTURE: EFFECTS OF SERUM

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Abstract
Effects of chicken- and fetal calf serum on secretion products of [rat] Sertoli cells were investigated. At day 5 or 6 of culture, Sertoli cells incubated with 1% chicken serum secreted significantly more androgen binding protein (ABP) than cells cultured in media without chicken serum. This effect of chicken serum was smaller than that of chicken serum together with FSH [follitropen] and testosterone. Addition of 1% chicken serum or fetal calf serum stimulated the incorporation of [3H]leucine in secreted proteins by Sertoli cells. Secretion of estradiol-17.beta. (E2) was stimulated by addition of fetal calf serum. Chicken serum did not stimulate the secretion of E2 even in the presence of extra added testosterone. The nature of the compounds in the sera responsible for these effects is unknown, but the effects cannot simply be explained by the presence of FSH or androgens. Compounds other than FSH and androgens may influence Sertoli cell function. Evidence was obtained that a serum component with a MW of less than 5000 may be involved in the stimulation of E2 production.

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