Improved Photostable FRET-Competent Biarsenical−Tetracysteine Probes Based on Fluorinated Fluoresceins
- 29 August 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 128 (37) , 12040-12041
- https://doi.org/10.1021/ja063212q
Abstract
We have developed fluoro-substituted versions of the biarsenical−tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensitivity, higher absorbance and quantum yield than FlAsH, and F4FlAsH adds a new color to the palette of biarsenical dyes. The two probes also provide a new FRET pair with a larger Ro value (54 Å) than any previously obtained with biarsenical dyes.Keywords
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