Changes in the heterogeneity of ribulosebisphosphate carboxylase–oxygenase in winter rye induced by cold hardening

Publisher Website
Google Scholar
Abstract
The quaternary structures of ribulose-1,5-bisphosphate carboxylase-oxygenase from cold-hardened and unhardened ''Puma'' rye were examined by 2-dimensional gel electrophoresis according to the method of O''Farrell. Evidently, major changes in charge heterogeneity occur in the large subunit of this enzyme during growth at cold-hardening temperatures. The extent of charge heterogeneity decreased upon adaptation of ''Puma'' rye to cold-hardening temperatures. In addition to charge heterogeneity, MW heterogeneity was also evident in the large subunit polypeptides of the enzyme from cold-hardened and unhardened ''Puma'' rye.