Transformation of UV-hypersensitive Chinese Hamster Ovary Cell Mutants with UV-irradiated Plasmids
- 1 January 1988
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 53 (2) , 249-260
- https://doi.org/10.1080/09553008814550601
Abstract
Transfection of UV-hypersensitive, DNA repair-deficient Chinese hamster ovary (CHO) cell lines and parental, repair-proficient CHO cells with UV-irradiated pHaprt-1 or pSV2gpt plasmids resulted in different responses by recipient cell lines to UV damage in transfected DNA. Unlike results that have been reported for human cells, UV irradiation of transfecting DNA did not stimulate the genetic transformation of CHO recipient cells. In repair-deficient CHO cells, proportionally fewer transformants were produced with increasing UV damage than in repair-proficient cells in transfections with the UV-irradiated hamster adenine phosphoribosyltransferase (APRT) gene contained in plasmid pHaprt-1. However, transfection of CHO cells with UV-irradiated pSV2gpt resulted in neither decline in transformation frequencies in repair-deficient cell lines relative to repair-proficient cells nor stimulation of genetic transformation by UV damage in the plasmid. Blot hybridization analysis of DNA samples isolated from transformed cells showed no dramatic changes in copy number or arrangement of transfected plasmid DNA with increasing UV dose. We conclude that the responses of recipient cells to UV-damaged transfecting plasmids depend both on the type of recipient cell and the characteristics of the genetic sequence used for transfection.Keywords
This publication has 27 references indexed in Scilit:
- High frequency of homologous recombination in mammalian cells between endogenous and introduced SV40 genomesCell, 1985
- Effect of DNA damage on the expression of the chloramphenicol acetyltransferase gene after transfection into diploid human fibroblastsNucleic Acids Research, 1985
- Correlation of gene expression and transformation frequency with the presence of an enhancing sequence in the transforming DNA.Molecular and Cellular Biology, 1984
- High-frequency structural gene deletion as the basis for functional hemizygosity of the adenine phosphoribosyltransferase locus in Chinese hamster ovary cells.Proceedings of the National Academy of Sciences, 1983
- Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.Molecular and Cellular Biology, 1982
- Isolation of transforming DNA: Cloning the hamster aprt geneCell, 1980
- Mutagenicity testing in mammalian cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1980
- Large-scale isolation of UV-sensitive clones of CHO cellsSomatic Cell and Molecular Genetics, 1980
- A simple method for the preparation of large quantities of pure plasmid DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973