Detection of Furfural and 2-Furoic Acid in Bacterial Cultures by High Pressure Liquid Chromatography

Abstract
The Furfural and 2-furoic acid present in bacterial cultures were extracted and detected by High pressure Liquid Chromatogrphy (HPLC). Using the methanol/water solvent (70/30), the column, μ Bondapak C18 (Waters Associates) seperated these compounds well. The detection was performed at 254 nm where binary mixtures were absorbed. This method provided a rapid and simultaneous detection for the conversion of furfural into 2-furoic acid followed by the utilization of 2-furoic acid during bacterial growth.

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