The storage of washed human erythrocytes in a protein‐free medium
- 2 January 1983
- journal article
- research article
- Published by Wiley in Transfusion
- Vol. 23 (1) , 8-10
- https://doi.org/10.1046/j.1537-2995.1983.23183147318.x
Abstract
Protein-free red blood cells were prepared by washing fresh citrate-phosphate-dextrose [CPD] anticoagulated whole blood and resuspending the cells in a medium containing saline, adenine, phosphate and glucose (APG). The protein concentration in the APG medium was reduced to 0.0024% of the original plasma protein. The 24-h red blood cell posttransfusion survival after 21 days of storage was 80%. Extracellular K concentrations were less than CPD-plasma control after 21 days of storage. ATP concentrations were lower after storage in APG, but intracellular ATP was not closely associated with posttransfusion survival. Hemolysis was slightly increased in the APG group. There are no insurmountable barriers to the use of proteinfree media for the storage of washed red blood cells.This publication has 6 references indexed in Scilit:
- Clinical Usefulness of Red Cells Preserved in Protein-Poor MediumsNew England Journal of Medicine, 1978
- Red Blood Cell Preservation in Protein‐Poor Media: I. Leukocyte Enzymes as a Cause of HemolysisTransfusion, 1978
- Storage of Erythrocytes in Artificial MediaTransfusion, 1971
- Effect of Resuspension Medium on in Vivo Survival and Supernatant Hemoglobin of Erythrocytes Preserved with GlycerolTransfusion, 1965
- Multiple Automatic Sequential AnalysisClinical Chemistry, 1964
- THE COLORIMETRIC DETERMINATION OF THE SERUM PROTEINSPublished by Elsevier ,1929