LacZ staining in paraffin-embedded tissue sections.
- 1 November 1996
- journal article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 44 (11) , 1323-1329
- https://doi.org/10.1177/44.11.8918907
Abstract
Femora and tibiae of rats carrying leukemia from a LacZ-marked acute promyelocytic leukemia-derived leukemic cell line (LT12NL15) were decalcified using EDTA and routinely embedded in paraffin. Sections were used to develop for the first time an immunostaining method for LacZ, employing catalyzed reporter deposition (CARD) based on the deposition of biotinylated tyramine. This method was used to study homing and adhesion of leukemic cells.Keywords
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