Isolation ofMycoplasma bovis from intact and microinjected preimplantation bovine embryos washed or treated with trypsin or antibiotics

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Abstract
Incubation of day 7 bovine embryos with 104 or 106 CFU/ml ofMycoplasma bovis (M. bovis) or microinjection ofM. bovis into the cells of day 7 embryos did not influence embryonic development.M. bovis was recovered from all embryos washed 10 times by a standard pipetting method or vortexed and pipeted 10 times.M. bovis was also recovered from zonae pellucidae removed and washed from microinjected embryos. Neither treatment with trypsin nor exposure of embryos to combinations of penicillin, streptomycin, lincomycin and spectinomycin, or gentamicin, tylosin, lincomycin, and spectinomycin, inactivatedM. bovis.