HPLC ANALYSIS OF TC-99M IMINODIACETATE HEPATOBILIARY AGENTS AND A QUESTION OF MULTIPLE PEAKS - CONCISE COMMUNICATION

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 21  (12) , 1180-1184
Abstract
The application of high-performance liquid chromatography (HPLC) to 99mTc-labeled iminodiacetate hepatobiliary agents is described. The method is sensitive to variations of phenyl ring structure and gives different retention times for the different agents. 99mTc-labeled N-(2,6-diethylacetanilide)iminodiacetate from different sources had 2 components in different amounts. It was determined that the 2nd component, which is minor at pH > 5, is major when prepared at pH 4.5 or less. Raising the pH results in rapid conversion of the 2nd component into the 1st. This observation was demonstrated in rat plasma and would be expected to occur when the radiopharmaceutical is injected i.v. The kinetics of appearance of the preparations in the bile when prepared at pH 4 and pH 6 were essentially the same.