Micropropagation method for a hybrid willow (Salix matsudana×albaNZ-1002)

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Abstract
A method is described for rapid in vitro multiplication of a hybrid willow (S. matsudana × alba NZ-1002). On Murashige and Skoog's medium (MS) containing benzylaminopurine (BAP) at 0.1 mg/l and naphthaleneacetic acid (NAA) at 0.2 mg/l a 4-fold shoot multiplication can be achieved in 4 weeks. The isolated shoots produced in this way, after being rooted on MS containing only NAA (0.2 mg/l) for 10 days, can be transplanted to pots with over 90% success. Of the various cytokinins tested for shoot multiplication BAP proved most effective, followed by kinetin. 2-isopentenyl adenine (2-ip) was ineffective at any of the tested levels (0.1-1.0 mg/l).

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