VI.—TOWARDS A SOLUTION OF THE GOLGI PROBLEM: RECENT DEVELOPMENTS IN CYTOCHEMISTRY AND ELECTRON MICROSCOPY

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Abstract
SYNOPSIS: The Golgi techniques cause the deposition of silver or osmium on the Dalton complex (an association of double membranes, vacuoles, and granules seen in electron micrographs of the cytoplasm of many kinds of cells). It is denied that the Dalton complex occurs in all kinds of cells. In the neurones of gastropods (and in many other cells of invertebrates) the Golgi techniques reveal lipid droplets having no relationship with the Dalton complex. It is uncertain what objects in the living cell are responsible for the production of the classical Golgi apparatus of the neurones of vertebrates.Some cells contain the Dalton complex and also phospholipid droplets; both these kinds of cytoplasmic inclusions are blackened by the Golgi techniques. Several cases are quoted in which two quite different objects in the same cell have both been called Golgi apparatus.The following appear to be the only cytoplasmic inclusions that occur regularly in all or nearly all kinds of cells: mitochondria, lipid droplets (very diverse in structure and chemical composition), endoplasmic reticulum, “small particles” of Palad.