RAPID ISOLATION OF FUNCTIONALLY INTACT PANCREATIC-ISLETS FROM MICE AND RATS BY PERCOLLTM GRADIENT CENTRIFUGATION
- 1 January 1980
- journal article
- research article
- Vol. 6 (2) , 87-89
Abstract
A discontinuous gradient of Percoll was used to effectively separate islets of Langerhans from collagenase digested pancreas from mice and rats. Percoll isolated islets and islets isolated by direct hand-picking from the pancreatic digest were maintained in tissue culture. Percoll isolation of islets did not affect glucose-stimulated insulin release from islets cultured for 1 week or the ability of islets from newborn rats to incorporate 3H-thymidine into DNA. Centrifugation in Percoll is an efficient method to isolate functionally intact pancreatic islets.This publication has 7 references indexed in Scilit:
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