Fusion of Spheroplasts and Genetic Recombination ofZymomonas mobilis

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Abstract
Spheroplasts of Z. mobilis (Z-6) were prepared by cultivating it in a hypertonic medium containing penicillin G or glycine at 30.degree. C for > 6 h. The reversion of spheroplasts to a bacillary form was observed at frequencies of 10-2 to 10-3 when spheroplasts were incubated on hypertonic solid agar plates overlaid with soft agar. Spheroplast fusion was attempted with polyethylene glycol 6000 by crossing 2 mutants lacking the ability to ferment sugars. Fusants were selected and obtained at high frequencies. The properties of a stable fusant are discussed.

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