Ex planta phytoremediation of trichlorophenol and phenolic allelochemicals via an engineered secretory laccase
- 13 June 2004
- journal article
- letter
- Published by Springer Nature in Nature Biotechnology
- Vol. 22 (7) , 893-897
- https://doi.org/10.1038/nbt982
Abstract
Plant roots release a range of enzymes capable of degrading chemical compounds in their immediate vicinity1,2. We present a system of phytoremediation ex planta based on the overexpression of one such enzyme, a secretory laccase. Laccases catalyze the oxidation of a broad range of phenolic compounds3, including polychlorinated phenols such as 2,4,6-trichlorophenol (TCP), that are among the most hazardous and recalcitrant pollutants in the environment4. We isolated a secretory laccase cDNA of LAC1, which is specifically expressed in the roots of Gossypium arboreum (cotton). Transgenic Arabidopsis thaliana plants overexpressing LAC1 exhibited enhanced resistance to several phenolic allelochemicals and TCP. The secretory laccase activity in these plants was responsible for the conversion of sinapic acid into a mono-lactone type dimer and for the transformation of TCP.Keywords
This publication has 28 references indexed in Scilit:
- Allelopathy and Exotic Plant Invasion: From Molecules and Genes to Species InteractionsScience, 2003
- Engineering tolerance and accumulation of lead and cadmium in transgenic plantsNature Biotechnology, 2003
- Laccase: new functions for an old enzymePhytochemistry, 2002
- Laccase Down-Regulation Causes Alterations in Phenolic Metabolism and Cell Wall Structure in PoplarPlant Physiology, 2002
- Rapid Total Destruction of Chlorophenols by Activated Hydrogen PeroxideScience, 2002
- Phytodetoxification of TNT by transgenic plants expressing a bacterial nitroreductaseNature Biotechnology, 2001
- Molecular cloning and functional identification of (+)‐δ‐cadinene‐8‐hydroxylase, a cytochrome P450 mono‐oxygenase (CYP706B1) of cotton sesquiterpene biosynthesisThe Plant Journal, 2001
- Identification of Glucosyltransferase Genes Involved in Sinapate Metabolism and Lignin Synthesis in ArabidopsisJournal of Biological Chemistry, 2001
- Phytoremediation: A clean transition from laboratory to marketplace?Nature Biotechnology, 1997
- An Arabidopsis mutant defective in the general phenylpropanoid pathway.Plant Cell, 1992