Specific Labeling of a Phosphate‐Transporting Protein from Rat‐Liver Mitochondria by [203Hg]Mersalyl
- 1 December 1979
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 102 (2) , 605-614
- https://doi.org/10.1111/j.1432-1033.1979.tb04278.x
Abstract
A highly specific labeling of a phosphate-transporting protein from rat-liver mitochondria was obtained with [203Hg]mersalyl under the following conditions: labeling of mitoplasts, isolated by the French-Press procedure; and washing of the isolated inner membranes with NaCl/octylglucoside. The concentration-dependent labeling of the protein with mersalyl showed a biphasic saturation which correlated well with the protection and inhibition of phosphate transport. The MW (31,000) and the amount of labeled carrier protein (24 nmol/g mitochondrial protein) at 100% protection correlated well with published data from the literature. Gel filtration of the labeled protein in the presence of non-ionic detergents showed a MW of at least twice of that obtained by gel electrophoresis in the presence of sodium dodecylsulfate.This publication has 40 references indexed in Scilit:
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