Vibriocidal Antibody Inhibition (VAI) Analysis: A Technique for the Identification of the Predominant Vibriocidal Antibodies in Serum and for the Detection and Identification of Vibrio Cholerae Antigens

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Abstract
Summary: The activity of vibriocidal antibody can be specifically inhibited by minute amounts of endotoxins from Vibrio cholerae. This observation has been applied in the development of a procedure called the Vibriocidal antibody inhibition test (VAIT) which can be adapted to serve as a sensitive assay for the detection, identification and quantification of V. cholerae antigens and, by Vibriocidal antibody inhibition (VAI) Analysis, for the identification of the predominant vibriocidal antibodies in human and rabbit sera. Acute phase sera from cholera patients were found to contain antigen-like inhibitory activity for the vibriocidal reaction. The significance of these findings is discussed.