Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector.
- 1 December 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (24) , 8404-8408
- https://doi.org/10.1073/pnas.82.24.8404
Abstract
A cDNA coding for human interleukin 2 (IL-2) was inserted into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. Cells infected with recombinant virus produced high levels of Mr 15,500 IL-2 polypeptide, the majority of which was secreted into the culture medium during infection. The recombinant IL-2 was able to stimulate the growth of an IL-2-dependent cell line. The N-terminal amino acid sequence of the insect-derived IL-2 was identical to that of natural IL-2. Thus, a mammalian signal peptide was recognized and properly removed in insect cells.This publication has 28 references indexed in Scilit:
- Amino acid sequence and post-translational modification of human interleukin 2.Proceedings of the National Academy of Sciences, 1984
- Biological Activity of Recombinant Human Interleukin-2 Produced in Escherichia coliScience, 1984
- Production of human beta interferon in insect cells infected with a baculovirus expression vector.Molecular and Cellular Biology, 1983
- Two types of glycoprotein precursors are produced by the simian rotavirus SA11Virology, 1983
- Structure and expression of a cloned cDNA for human interleukin-2Nature, 1983
- Heterogeneity of human T-cell growth factor(s) due to variable glycosylationMolecular Immunology, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- T Cell Growth Factor: Parameters of Production and a Quantitative Microassay for ActivityThe Journal of Immunology, 1978
- Long term culture of tumour-specific cytotoxic T cellsNature, 1977
- Established Insect Cell Line from the Cabbage Looper, Trichoplusia niNature, 1970