The Analysis of Melatonin, Tryptamine, and Serotonin by HPCE and CEC
- 1 November 1998
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography & Related Technologies
- Vol. 21 (19) , 2923-2934
- https://doi.org/10.1080/10826079808006876
Abstract
A capillary electrophoresis method has been developed for the analysis of melatonin, which depends on whether tryptamine and serotonin must be determined as well. If only a melatonin analysis is required, a bare capillary with a pH 4.41 buffer separates the analyte from the other two compounds, which are unresolved. If determination of all three species is necessary, a bare capillary with a pH 2.14 buffer is used. For better resolution of tryptamine and serotonin without the analysis of melatonin, a chemically modified capillary surface that lowers the electroosmotic flow is necessary.Keywords
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