Determination of Trazodone(R) and Its Metabolite, m-CPP, in Serum and Urine by HPLC

Abstract
A sensitive and rapid method for the analysis of trazodone (TZD) and its metabolite, 1-(m-chlorophenyl)piperazine (m-CPP), in the serum and urine of rabbits treated with the drug was developed. The assay requires extraction from the biological fluids with adequately buffered organic solvents followed by HPLC. The assay allows good reproducibility, fair recovery, and excellent linearity in the range of 0.6 to 10 µg/mL for TZD and 1.2 to 20 µg/mL for m-CPP.

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