Glycoproteins of herpes simplex virus type 2 as defined by monoclonal antibodies

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Abstract
Mouse monoclonal antibodies reacting with glycoproteins specificed by herpes simplex virus type 2 (HSV-2) were used to characterize the individual antigens in terms of structure, processing and kinetics of synthesis in [hamster kidney] BHK or [African green monkey kidney] Vero infected cells. The results provided a direct demonstration of the structural identity of the gA and gB proteins of HSV-2 as well as confirmation of the existence of type-specific and type-common domains within the gD molecule. With the exception of gC, processing of the viral glycoproteins differs to some extent in Vero and BHK infected cells, possibly as a result of different efficiency of glycosylation or different processing of underglycosylated and unglycosylated products in the 2 cell types. Apparently, individual HSV-2 glycoproteins are synthesized at greatly different times during the infectious cycle, possibly in response to their different roles in virus replication and assembly.