Gly429 is the major determinant of uncompetitive inhibition of human germ cell alkaline phosphatase by l-leucine
- 15 February 1991
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 274 (1) , 91-95
- https://doi.org/10.1042/bj2740091
Abstract
The catalytic activity of human placental alkaline phosphatase (PLAP) and germ cell alkaline phosphatase (GCAP) can be inhibited, through an uncompetitive mechanism, by L-Phe. GCAP is also selectively inhibited by L-Leu. Site-directed mutagenesis of five of the 12 residues which are different in PLAP and GCAP revealed that Gly429 is the primary determinant of GCAP inhibition by L-Leu, and Ser84 and Leu297 play a modulatory role in the inhibition.Keywords
This publication has 26 references indexed in Scilit:
- Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.Molecular and Cellular Biology, 1982
- HYPOPHOSPHATASIA (ADULT FORM) - QUANTITATION OF SERUM ALKALINE-PHOSPHATASE ISOENZYME ACTIVITY IN A LARGE KINDRED1980
- L-p-Bromotetramisole, a new reagent for use in measuring placental or intestinal isoenzymes of alkaline phosphatase in human serum.Clinical Chemistry, 1977
- L-leucine, a specific inhibitor of a rare human placental alkaline phosphatase phenotypeNature, 1976
- Perspectives on alkaline phosphatase isoenzymesThe American Journal of Medicine, 1974
- Studies on Alkaline PhosphataseEuropean Journal of Biochemistry, 1972
- L-Homoarginine. An organ-specific, uncompetitive inhibitor of human liver and bone alkaline phosphohydrolases.1972
- Organ-specific inhibition of human alkaline phosphatase isoenzymes of liver, bone, intestine and placenta; L-phenylalanine, L-tryptophan and L homoarginine.1971
- On the Mechanism of Inhibition of Intestinal Alkaline Phosphatase by l-PhenylalanineJournal of Biological Chemistry, 1966
- L-Phenylalanine : an Organ Specific, Stereo-specific Inhibitor of Human Intestinal Alkaline PhosphataseNature, 1963