ISOLATION AND CYTOCHEMICAL PROPERTIES OF HUMAN ENDOMETRIAL CELLS

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Abstract
Quantitative cytochemical methods were employed for the determination of some cellular properties of isolated endometrial cells. Two different methods have been used: an absorption microspectrophotometric method for the estimation of Feulgen-DNA and a microfluorometric technique for the assessment of glycogen. The two methods have been evaluated with respect to their specificity when applied to isolated human endometrial cells. The variation in endometrial DNA and glycogen during the menstrual cycle was studied in isolated cells taken from normally menstruating women. During the first part of the proliferative phase from cycle day (c. d.) 1 to c. d. 10–11 the Feulgen-DNA values in the endometrial cells remained at a constant level, corresponding to the pre-synthesizing phase (G1). However, in the samples obtained from cycle days 14, 16–18, and 19–23 the cells were mainly in the DNA-synthesizing phase (S), revealing a higher amount of Feulgen-DNA per nucleus than in the early proliferative phase. By c. d. 27 a statistically significant drop in Feulgen-DNA per cell nucleus was found and the endometrial cells were again in G1-phase. The deposit of glycogen showed a gradual increase from c. d. 1 to c. d. 16–18, with a slope of 0.0079 ± 0.0022. This was followed by a significant decrease between c. d. 16–18 and c. d. 27 with a slope of −0.0139 ± 0.0042. Stromal glycogen remained low and constant throughout the entire menstrual cycle.

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