A CRITICAL COMPARISON OF THE T-1824 DYE AND IODINATED ALBUMIN METHODS FOR PLASMA VOLUME MEASUREMENT 1

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Abstract
Plasma volumes were detd. simultaneously with T-1824 dye and iodinated albumin in 28 subjects. The mean plasma volume with iodinated albumin was 3255 cc. and with the dye method 3314 cc. This difference was not significant. The correlation coeff. was 0.927. No significant difference between the volumes calculated from a single 10 min. blood sample and volumes calculated from an extrapolation of 6 samples taken at 10 min. intervals for 1 hr. was found with either iodinated albumin or T-1824 dye. In 10 patients the plasma volume was detd. with both methods simultaneously, before and immediately after the admin. of 1000 cc. of polyvinylpyrrolidone (PVP). The increase in plasma volume as measured by the 2 methods was not significantly different. The decrease in plasma concn. of iodinated albumin and T-1824 dye during a 1 hr. period after injn. was compared and no significant difference was found. The mean per cent change was 11.5 for iodinated albumin and 11 for the dye. The cervical portion of the thoracic duct was cannulated in a 27-year- old man, during a radical neck dissection. Samples of lymph and plasma were obtained at 10- to 20-min. intervals for 140 min. after the intraven. injection of iodinated albumin. The per cent of the injected dose per 100 ml. of lymph was 0.18 at 30 min. The plasma/lymph ratio was 40.0, 14.3, 3.0, and 2.1 at 10, 30, 65, and 135 min., respectively. The amt. of tagged albumin appearing in the thoracic duct lymph during the detn. of the plasma volume is small and does not significantly influence the measurement of plasma volume. Plasma volume measurements made with T-1824 dye are as valid as those made with iodinated albumin.