Effect of Various Factors on the Efficiency of Plating and Plaque Morphology of some Streptomyces Phages

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Abstract
The effect of medium, temperature, and amount of inoculum on the efficiency of plating (EOP) and plaque morphology of 6 Streptomyces phages was investigated. NaCl (0.5%) exhibited an inhibitory effect on the formation of plaques by the 2 small plaque-producing phages. The other 4 phages yielded about the same EOP on all 5 media tested. Two phages did not produce plaques at 37[degree], but did at 22[degree], 28[degree], and 32[degree]; one phage produced plaques at 22[degree] and 28[degree], but not at 32[degree] and 37[degree]. The other 3 phages formed plaques between 22[degree] and 37[degree]. Increasing amounts of inoculum led to a decrease in plaque counts. Conditions (medium, temperature) which are suitable for one phage-host system might be quite unsuitable for another system comprising the same phage.

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