Thin-Layer Chromatographic Separation of14C-Labeled Metabolites from Photosynthate

Abstract
A method for separating 14C-labeled metabolites (including key intermediates of the glycolate pathway) from plant extracts by thin-layer chromatography is reported. The method provides for rapid and convenient determination of the 14C-content of metabolites in leaf samples obtained during kinetic investigations of photosynthesis with 14CO2.