Rapid transport of the acidic phosphoproteins ofPlasmodium berghei andP. chabaudi from the intraerythrocytic parasite to the host membrane using a miniaturized fractionation procedure
- 1 January 1992
- journal article
- Published by Springer Nature in Zeitschrift Fur Parasitenkunde-Parasitology Research
- Vol. 78 (3) , 193-200
- https://doi.org/10.1007/bf00931726
Abstract
A miniaturized procedure for the separation of the host erythrocyte membrane from malarial parasites based on saponin lysis and density-gradient centrifugation with Percoll is described. The procedure requires only 20–35 μl packed infected erythrocytes, is simple to perform, needs no sophisticated equipment, and can be completed in Plasmodium berghei andP. chabaudi were rapidly transported from the parasite to the erythrocyte membrane immediately after their synthesis. The rapid export of these acidic phosphoproteins from the parasite distinguishes them from other proteins exported by the malarial parasite.Keywords
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