Crosslinking of double-helical nucleic acids with a photoreactive analog of ethidium
- 21 June 1983
- journal article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 22 (13) , 3226-3231
- https://doi.org/10.1021/bi00282a029
Abstract
Intercalation of the ethidium analogue 3,5-diazido-5-ethyl-6-phenylphenanthridinium into double helices followed by irradiation with blue or ultraviolet light results in cross-linking between the two strands with an efficiency around 30% for DNA, RNA, and DNA-RNA hybrids. Details of this reaction and a convenient synthesis of the ethidium analogue are described. Stable tertiary structure in RNA impedes intercalation and thus reduces the efficiency of cross-linking. In contrast to the ethidium derivative, various acridine diazides show little or no cross-linking ability.Keywords
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