Selenium suppresses the metabolism of benzo[a]pyrene by rat-liver extracts, and exerts a dual effect on its mutagenicity
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in Xenobiotica
- Vol. 14 (12) , 893-902
- https://doi.org/10.3109/00498258409151488
Abstract
1. Liver homogenates from rats injected with 3 -methylcholanthrene were employed for metabolism of benzo[a]pyrene (BP) and in assays of aryl hydrocarbon hydroxylase (AHH) activity in vitro. 2. Sodium selenite inhibited AHH activity to a maximum of ∼70%. It suppressed the overall metabolism of benzo[a]pyrene; a distinct reduction in the products was evident on h.p.l.c. analysis. 3. Sodium thiosulphate also inhibited AHH activity by ∼ 47%. Inclusion of S2O2-3 and SeO2-3, in combination, led to a cumulative inhibition of 87%. 4. The mutagenicity of BP in the Salmonella auxotroph reversion system (Ames test) was enhanced by SeO2-3 at concentrations below 0.2 mM. Above this level a significant anti-mutagenic effect was observed.This publication has 24 references indexed in Scilit:
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