Selective retention of organic phosphate esters and phosphonates on aluminium oxide
- 1 May 1986
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 6 (5) , 477-483
- https://doi.org/10.1007/bf01116139
Abstract
Compounds containing the −PO3H2 function, such as monoesters of phosphoric acid and phosphonic acids, specifically bind to aluminium oxide in aqueous solution under experimental conditions where non-phosphorylated compounds are completely desorbed. The bound organic phosphate can be specifically displaced by aqueous solution of inorganic phosphates thus allowing their separation or detection by a technique similar to that of affinity chromatography. The consequences of this finding for phosphate compound biochemistry are discussed.This publication has 14 references indexed in Scilit:
- Protein kinasesPublished by Elsevier ,1984
- The Protein Phosphatases Involved in Cellular Regulation. 1. Classification and Substrate SpecificitiesEuropean Journal of Biochemistry, 1983
- The role of protein phosphorylation in neural and hormonal control of cellular activityNature, 1982
- Specific sulfonation of tyrosine, tryptophan and hydroxy-amino acids in peptidesBiochimica et Biophysica Acta (BBA) - Protein Structure, 1979
- [3] Determination of proteolytic activities on casein substratesPublished by Elsevier ,1976
- A new simple method for separation of adenosine 3′,5′-cyclic monophosphate from other nucleotides and its use in the assay of adenyl cyclaseAnalytical Biochemistry, 1971
- Separation of cyclic 3′,5′-nucleoside monophosphates from other nucleotides on aluminum oxide columns application to the assay of adenyl cyclase and guanyl cyclaseAnalytical Biochemistry, 1971
- Thin-Layer ChromatographyPublished by Springer Nature ,1969
- Infrared and Gravimetric Study of the Surface Hydration of γ-AluminaThe Journal of Physical Chemistry, 1965
- [11] Separation of proteins by use of adsorbentsPublished by Elsevier ,1955