SIMPLIFIED APPROACH TO CONFIDENCE-LIMITS IN RADIOIMMUNOASSAY

  • 1 January 1980
    • journal article
    • research article
    • Vol. 26  (6) , 763-765
Abstract
A simple method of calculating confidence limits for radioimmunoassay data is presented. The method involves the use of the within-assay variation in a dose estimate of 3 routine quality-control specimens, measured in repeated assays, to estimate the confidence limits for results on unknown samples. Results for control specimens are combined by calculating the unique quadratic curve fitting a graph of within-assay SD vs. mean value for each control. This method requires no special data accumulation or advanced computing equipment. For cortisol, lutropin and thyroxine radioimmunoassays, confidence limits calculated in this way were compared with those calculated from the variance of the response variable B/BO in repeated standard curves. Both methods agree well with actual limits observed when plasma pools containing a wide range of hormone concentrations are assayed repeatedly.

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