Further Studies on Urease Production byProteusand Related Organisms

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Abstract
Conditions of the test for urease production by Proteus organisms were investigated. The volume of medium surface to volume ratio of the medium and the size of inoculum may increase or decrease the speed of urease production. Changes in the buffer conc. markedly alter the time required to detect urease production and changes in the temp. markedly increase or decrease the speed of urease production. At 20[degree] C the first, medium and last P. morganii strains were positive in 32, 58 and 130 hrs., respectively; at 42.5[degree] C a positive reaction was obtained in 4, 6 and 8 hrs., respectively. In weakly buffered medium'' the relationship of Proteus to other spp. and genera in the Enterobacteriaceae, with respect to urease production, was studied. A rapid urease test is described.