Determination of Serum Thyroxine after Dissociation from Thyroxine-Binding Globulin in Alkaline Solution and Adsorption on Dextran-Coated Charcoal
Open Access
- 1 February 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 21 (2) , 216-220
- https://doi.org/10.1093/clinchem/21.2.216
Abstract
A competitive protein-binding procedure for determining serum thyroxine is described in which thyroxine is first dissociated from serum thyroxine-binding globulins in an alkaline solution. After binding equilibration, the unbound thyroxine is separated by dextran-coated charcoal. The procedure takes only 100 µl of serum, requires no evaporation, and is sensitive and reproducible. Results by this procedure correlate well with those for a generally accepted method.Keywords
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