Presence of Both Photosystems in Guard Cells of Vicia faba L

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Abstract
A new procedure was reported for high-yield isolation of guard cell protoplasts from V. faba L. Delayed light emission and P700 content plus absorption and fluorescence emission spectra of the protoplast extracts were reported. Both photosystems apparently were present. The presence of photosystem II and the absence of the reductive-step enzyme [NADP-glyceraldehyde-phosphate dehydrogenase] of the Calvin-Benson Cycle (Outlaw et al.) in a cell had no precedent in the literature. Evidently, noncyclic photosynthetic electron flow was an environmental sensor which caused stomata to remain open in light.