Antigen-Induced Deoxyribonucleic Acid Synthesis in Mouse Lymphocytes

Open Access
Publisher Website
Google Scholar
Abstract
Employing the model of highly specific hapten-carrier conjugate-induced deoxyribonucleic acid (DNA) synthesis in primed mouse spleen cells, we have investigated the role of various cell populations in generating this response. The depletion of adherent cells results in no loss of cell viability and no decrease in dinitrophenyl-keyhole limpet hemocyanin-induced replication. After depletion of thymus-derived (T) lymphocytes with anti-ϑ serum and complement, the response of DNP-KLH-primed spleen cells to DNP-KLH was reduced by 66%. On the other hand, a population of DNP-KLH-primed spleen cells depleted of immunoglobulin-bearing B lymphocytes by treatment with rabbit anti-mouse immunoglobulin antibodies and complement developed a response to DNP-KLH only 59% of that seen with normal cells. These findings indicate that DNP-KLH antigen-induced DNA synthesis is a mixed cell response, with replication occurring largely in the B lymphocyte population. T lymphocytes also form a portion of the replicating pool and may be more crucially required for a triggering step in the antigen-induced DNA synthetic response in the mouse. The cellular components of KLH and DNP-d-GL-induced DNA synthesis in normal and DNP-KLH-primed mouse spleen cells were also investigated in detail.

This publication has 0 references indexed in Scilit: