Dynamics of Salmonella Isolation with Modified Semi-Solid Rappaport-Vassiliadis Medium
- 1 August 1987
- journal article
- Published by Elsevier in Journal of Food Protection
- Vol. 50 (8) , 658-661
- https://doi.org/10.4315/0362-028x-50.8.658
Abstract
The effect of low Salmonella cell concentrations in combination with high numbers of competitive flora after pre-enrichment on subsequent isolation with motility enrichment in modified semisolid Rappaport-Vassiliadis (MSRV) was studied. Salmonella was always detected on MSRV if the cell concentration in the preenrichment culture was at least 60 per ml, even if they were outnumbered by competitive bacteria by a factor of 107. In a total of 52 experiments, Salmonella detection on MSRV directly after preenrichment was successful in 46 tests. Combination of direct motility enrichment with additional motility enrichment after 8 h in tetrathionate brilliant green (TBG) broth resulted in 49 positive tests, compared to only 33 positive results after enrichment in TBG for 24 h with subsequent plating on selective agars.Keywords
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