Freezing of Whole Blood

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Abstract
From earlier expts., the author indicates it is possible to freeze whole blood in a solid mass and thaw it without appreciable hemolysis. Accordingly, more recently, 150 specimens of blood have been frozen and thawed under varying conditions of temp., heat dissipation (affecting the time of freezing), concn. of electrolytes, pH, concn. of diffusible and nondiffusible sugars (affecting the size of the erythrocytes), etc. Results on approx. 100 specimens of blood indicate that freezing and thawing were accomplished with a resulting hemolysis of less than 1% of the cells. Preservation of these specimens has been satisfactory for varying time periods up to 1 month.

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