The measurement of glucuronide synthesis by tissue preparations

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Abstract
Small amts. of o-amino-phenylglucuronide can be detd. colorimetrically after diazotizing and coupling with naphthylethylenediamine. Interference with the color reaction by comparatively large amts. of o-aminophenol can be excluded if the color is developed at pH 2.25 and oxidation of o-aminophenol is avoided when ascorbic acid is present. Trichloroacetic acid solns. brought to pH 2.25 precipitate proteins if phosphate buffer at the same pH is also present, permitting color development without further pH adjustment. The color reaction has been applied to the measurement of glucuronide synthesis by mouse-liver slices. Rates of synthesis of the order of 0.7 mg. o-aminophenol conjugated/g. dry wt. of liver/hr. were obtained at the opt. concn. of 0.0025%.

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