Positive-Contrast Imaging in the Rabbit Hind-Limb of Transplanted Cells Bearing Endocytosed Superparamagnetic Beads
- 1 December 2006
- journal article
- Published by Elsevier in Journal of Cardiovascular Magnetic Resonance
- Vol. 8 (6) , 817-823
- https://doi.org/10.1080/10976640600777819
Abstract
Positive-contrast methods can augment negative-contrast methods for CMR evaluation of transplanted cell fate in vivo. This study correlated positive-contrast signal characteristics in the rabbit hind-limb with xenograft cell number (rat fibroblasts bearing endocytosed 1 microm Bang's particles) at different stages of Gd-DTPA administration and 1 mm2 in-plane resolution (2 minute scans, 1.5 Tesla). Linear regressions modeled satisfactorily the cell number dependencies of signal-to-noise (SNR) and area-of-enhancement (R > 0.83), over approximately a 50-fold fluctuation in cell number to a lowest detected limit of 25k cells during Gd-DTPA administration. Gd-DTPA administration elevated the slopes of parameter regressions by four or more standard deviations.Keywords
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