DETECTION OF PROTEOLYSIS BY SODIUM DODECYL SULFATE POLYACRYLAMIDE GEL ELECTROPHORESIS: A DEMONSTRATION OF PROTEIN HYDROLYSIS AND ELECTROPHORESIS FUNDAMENTALS
- 1 June 1986
- journal article
- research article
- Published by Hindawi Limited in Journal of Food Biochemistry
- Vol. 10 (2) , 83-91
- https://doi.org/10.1111/j.1745-4514.1986.tb00091.x
Abstract
A laboratory exercise is presented for evaluating the action of proteolytic enzymes on the proteins BSA and two types of catalase. The proteins are incubated with papain and bromelain-based meat tenderizers and the digestion products are analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The exercise demonstrates both electrophoresis fundamentals and the manner by which proteases function.This publication has 3 references indexed in Scilit:
- Effect of deglycosylation on the stability of Aspergillus niger catalaseArchives of Biochemistry and Biophysics, 1981
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970