A histochemical analysis of polyanionic compounds found in the extracellular matrix encountered by migrating cephalic neural crest cells
- 1 April 1980
- journal article
- research article
- Published by Wiley in The Anatomical Record
- Vol. 196 (4) , 401-412
- https://doi.org/10.1002/ar.1091960405
Abstract
Neural crest cells destined to form craniofacial primordia initially are “seeded” into and subsequently migrate through the extracellular matrix (ECM) of a cell free space (CFS) between the surface ectoderm and the underlying mesoderm. Utilizing histochemical procedures for polyanionic compounds, we have demonstrated that both sulfated and nonsulfated glycosaminoglycans (GAG) are present in the CFS of the cephalic region of the chick embryo and that their distribution and structural organization vary with the passage of neural crest or mesodermally derived (MD) mesenchymal cells through it. In stages 7 and 8 embryos a predominance of fine filamentous strands composed primarily of nonsulfated, carboxyl-rich GAG is seen spanning intercellular spaces between adjacent tissues and MD mesenchymal cells. In older embryos (stages 9 and 10) much of the filamentous material is replaced by coarse fibrillar strands or amorphous material which coats the surfaces of MD mesenchymal and neural crest cells as they invade the CFS. Using enzymatic digestions (Streptomyces and testicular hyaluronidase) and the critical electrolyte concentration procedure, data suggest that the fine filamentous matrix onto which the neural crest cells migrate consists mainly of hyaluronate with lesser amounts of chondroitin and some sulfated GAG present. The coarse fibrillar matrix that appears after passage of either neural crest or MD mesenchymal cells through the original CFS contains strongly sulfated polyanionic material, predominantly chondroitin sulfates A, C. Since GAG is located ubiquitously within the ECM of embryos at various stages, the role of GAG, if any, in the transfer of developmental information may be of a general nature (ie, stimulus of motility) rather than of specific morphogenetic cues (for specific differentiation into craniofacial primordia).This publication has 36 references indexed in Scilit:
- Distribution and synthesis of glycosaminoglycans during quail neural crest morphogenesisDevelopmental Biology, 1978
- Analysis of glycosaminoglycans within the extracellular environments encountered by migrating neural crest cellsDevelopmental Biology, 1978
- Structural analyses on the matrical organization of glycosaminoglycans in developing endocardial cushionsDevelopmental Biology, 1978
- Glycosaminoglycan synthesis in the chick gastrulaDevelopmental Biology, 1976
- An analysis of the migratory behavior of avian cephalic neural crest cellsDevelopmental Biology, 1975
- Glycosaminoglycan synthesis by the early embryonic chick heartDevelopmental Biology, 1973
- THE EFFECT OF DIGESTION WITH STREPTOMYCES HYALURONIDASE UPON CERTAIN HISTOCHEMICAL REACTIONS OF HYALURONIC ACID-CONTAINING TISSUESJournal of Histochemistry & Cytochemistry, 1973
- The fine structure of perinotochordal microfibrils in control and enzyme‐treated chick embryosJournal of Anatomy, 1971
- Sulfated extracellular matrix production in the embryonic heart and adjacent tissuesJournal of Experimental Zoology, 1970
- Developing boundary (basement) membranes in the chick embryoThe Anatomical Record, 1967