Measurement of free and free-plus-peptide hydroxyproline fractions in plasma.
- 1 February 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 24 (2) , 204-207
- https://doi.org/10.1093/clinchem/24.2.204
Abstract
Plasma hydroxyproline fractions--free-plus-peptide and free--were determined by a method involving centrifugal ultrafiltration and the procedure of Kivirikko et al. [Anal. Biochem. 19, 249 (1967)]. Within- and between-day precision (CV) for the combined fractions was 4--5% and 2.8--6%, respectively. Mean analytical recoveries of free hydroxyproline were 95.2% (87--109%) in the normal range, and 97% (91--108%) in the above-normal range. Normal ranges for plasma hydroxyproline fractions, based on data for 11 men and 19 women, were free-plus-peptide, 7.5--25.3; free, 4.9--22.7; and peptide (calculated), 0.1--5.1 mumol of hydroxyproline per litre, respectively. These ranges showed no sex-related differences and were gaussian in distribution. A factor was derived to transform data on concentrations in ultrafiltrate into plasma concentrations.This publication has 4 references indexed in Scilit:
- Catabolism and Excretion of Free Hydroxyproline in Infancy1Journal of Clinical Endocrinology & Metabolism, 1967
- Modifications of a specific assay for hydroxyproline in urineAnalytical Biochemistry, 1967
- Hydroxyproline in the Serum and Urine Normal Values and Clinical SignificanceActa Medica Scandinavica, 1966
- Value of Urine and Serum Hydroxyproline in the Diagnosis of Thyroid DiseaseJournal of Clinical Endocrinology & Metabolism, 1965