Enzymatic Saccharification of Cellulosic Materials after Hydrothermolysis and Organosolv Pretreatments

Abstract
Different cellulose samples as well as lingo-cellulosic materials which had been pretreated by either organosolv or hydrothermolysis processes were tested as to their degradability by a crude cellulase preparation from Trichoderma viride. The glucose concentrations in hydrolysate samples were determined by HPLC End-product inhibition of the cellulase complex by glucose and cellobiose was also studied, as were inhibitory effects caused by lignin- and hemicellulose-derived pretreatment side-products.