A Study on Traumatic Lumbar Punctures

Abstract
Spectrophotometric methods for estimating oxyhemoglobin and bilirubin and oxyhemoglobin of the red blood cells in hemorragic cerebrospinal fluid have been studied and the technics described. Three fractions are determined directly the oxyhemoglobin and bilirubin the bilirubin alone of the supernatant fluid and the oxyhemoglobin of the red cell precipitate. Insignificant hemolysis developed in hemorrhagic cerebrospinal fluid at room temperature and even less at 4[degree] C. A spinal fluid which contains 360 red blood cells per mm appears grossly clear. Fall in red cell count in the serial tubes is a reliable indication of a traumatic lumbar puncture.

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