Artificial Infestation of Corn with Western Corn Rootworm Eggs in Agar-Water12

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Abstract
The following 4 aspects of the efficacy of the wet method (suspension of eggs in agar-water) and the earlier dry method (mixture of eggs in dry fine soil) for infesting corn with eggs of Diabrotica vergifera LeConte were compared: homogeneity of mixtures; viability of eggs after extended periods in the mixtures; results of artificial field infestation of corn; and time requirement for preparing the mixtures. Results indicated that the agar-water method gave a much more homogeneous mixture, methods had no adverse effect on egg hatch when stored at 10°C for up to 30 days, there was no significant difference in mean numbers of corn rootworms per plant and mean root damage ratings between the wet and dry methods of field infestation of corn, and the wet method can be prepared in half of the time and would save much time.

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