Measurement of kinase activation in single mammalian cells

1 March 2000

journal article
research article
Published by Springer Nature in Nature Biotechnology

Vol. 18 (3) , 309-312
https://doi.org/10.1038/73760

Abstract

We demonstrate a new method for the simultaneous measurement of the activation of key regulatory enzymes within single cells. To illustrate the capabilities of the technique, the activation of protein kinase C (PKC), protein kinase A (PKA), calcium-calmodulin activated kinase II (CamKII), and cdc2 protein kinase (cdc2K) was measured in response to both pharmacological or physiological stimuli. This assay strategy should be applicable to a broad range of intracellular enzymes, including phosphatases, proteases, nucleases, and other kinases.

Keywords

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