B- and T-Cell Markers on Canine Lymphosarcoma Cells2

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Abstract
Presumptive T- and B-cell populations were detected in normal canine lymphoid tissue and in 39 cases of spontaneous canine lymphosarcoma. Rosette-forming techniques with indicator erythrocytes were used to detect surface immunoglobulin, receptors for human erythrocytes (E), receptors for complement (EAC), and the Fc portion of immunoglobulin (EA). The sum of E + EAC rosette-forming cells could account for nearly all the lymphoid cells of the spleen (98%) but only 70% of lymph node cells. Only a small percentage of normal thymocytes formed E-rosettes. Phytohemagglutinin stimulation dramatically increased the number of E-rosette-forming thymocytes (RT-cells), though a major population remained nonrosetting (NRT-cells). Most multicentric cases and the single alimentary case of lymphosarcoma had B-cell properties. T-cell, possible null-cell, and double-labeled T + B-cell multicentric cases were also found. Tumors with mixtures of T- and B-cells consisted of both morphologically normal and neoplastic cells. Of the 4 cases of thymic lymphosarcoma described, 2 had RT-cell characteristics and 2 had NRT-cell characteristics. An association was found between skin infiltration and T-cell tumors. Two cases of acute lymphoblastic leukemia were also observed; the first was associated with a thymic mass, whereas the second case showed extensive bone marrow involvement. Both leukemias consisted of cells devoid of surface receptors.

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