Use of Radioimmunoassays to Determine the Concentration of Streptococcal Group-Specific Antibodies in Rabbit Antisera
- 1 January 1975
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 114 (1_Part_1) , 59-63
- https://doi.org/10.4049/jimmunol.114.1_part_1.59
Abstract
A radioimmunoassay was developed for the quantitation of antibodies to the Group A, A-variant, and C carbohydrates in rabbit streptococcal antisera. The assay employs radiolabeled purified Group carbohydrate which has been tyrosylated to allow for incorporation of 125I. This assay has an advantage over the quantitative precipitin test because it measures non-precipitating antibody in addition to precipitating antibody. Furthermore, 7S anti-IgG in rabbit antisera give a falsely elevated value for the antibody concentration in the quantitative precipitin test. This did not occur with the radioimmunoassay. The assay described is reproducible, sensitive, and uses little antisera.This publication has 0 references indexed in Scilit: