A selective screen for transposable element mobilization in drosophila melanogaster

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Abstract
A selective system is described that provides a simple and sensitive assay for the detection and analysis of induced mobile element transpositions in Drosophila melanogaster. The system will detect a single event in samples greater than 106 and thus provides a eucaryotic assay system for monitoring the induction of transposition by a variety of agents including, but not limited to, chemical carcinogens and toxins, ionizing radiation, and various environmental pollutants. The experimental system focuses on an X‐linked rosy+ transposon and a conditional lethal system that permits the detection of a single transposition event in very large samples. The results of a pilot experiment utilizing this system are presented.